Primož Šket

Ph.D.

primoz.sket@ki.si

(01) 47-60-223

Primož Šket was born on December 20, 1977, in Ljubljana, Slovenia. He received his B.Sc. degree in organic chemistry at University of Ljubljana, Faculty of Chemistry and Chemical Technology in 2001 with thesis entitled: "Synthesis of reagents for asymmetric electrophilic bromination on the basis of camphor". From 2002 to 2005 he was a Ph.D. student working at the Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia under supervision of Prof. Dr. Janez Plavec. In 2005 he received his D.Sc. in chemistry at the same University with Ph.D. thesis entitled: "NMR studies of metal ion binding inside G-quadruplex DNA structures". Since 2009 he works as a research associate at the National Institute of Chemistry. For his Ph.D. thesis he got Krka’s prize and Pregl’s prize of National Institute of Chemistry for the best Ph.D. in the field of chemistry and relative sciences in 2006. Scientifically, he has been involved in various aspects of NMR spectroscopy of nucleic acids since 2002. During his PhD work and after he has focused on 3D G-quadruplex structures determination and on studies of their metal ion interactions. With the use of NMR spectroscopy he has shown that the folding topologies of G-quadruplex structures critically depend on the sequence details as well as nature of cations present in solution. In the year 2011, at international NMR conference Euromar 2011, he got for his poster one of the prizes titled Goethe-BMRZ Poster Prize. He devotes a lot of time to the study of the compounds in the solid, particularly active pharmaceutical ingredients, by means of solid-state NMR spectroscopy. Dr. Šket is a co-author of 47 publications published in scientific journals with high impact factors. He was co-organizer of several scientific meetings. Primož Šket is also a supervisor and co-mentor at diplomas and master thesis. Since 2017 Primož Šket is mentor to a young researcher.

Research interests of P. Šket include various aspects of the use of NMR spectroscopy in the field of the study of 3D structures of nucleic acids and its interaction with metal ions. At this work, in addition to NMR spectroscopy I am using DNA / RNA Synthesizer, UV spectroscopy, CD spectroscopy. Investigations of compounds in solid, especially pharmaceutical active ingredients by the use of NMR spectroscopy. Study of intermolecular interactions by NMR spectroscopy in solution and solid state. Determination of the crystallinity and amorphousness of solid samples. Determination of the impurities by NMR spectroscopy.

SELECTED PUBLICATIONS

P. Sket and J. Plavec
Not all G-quadruplexes Exhibit Ion-Channel-like Properties: NMR Study of Ammonium Ion (Non)movement within the d(G₃T₄G₄)₂ Quadruplex
J. Am. Chem. Soc. 2007, 129 (28), 8794-8800.

S. Ceru, P. Sket, I. Prislan, J. Lah and J. Plavec
A new pathway of DNA G-quadruplex formation
Angew. Chem. Int. Ed. 2014, 53 (19), 4881-4884.
Web access at Wiley Online Library

P. Sket, J. Pohleven, A. Kovanda, M. Stalekar, V. Zupunski, M. Zalar, J. Plavec and B. Rogelj
haracterization of DNA G-quadruplex species forming from C9ORF72 G4C2-expanded repeats associated with amyotrophic lateral sclerosis and frontotemporal lobar degeneration
Neurobiol. Aging 2015, 36 (2), 1091-1096.
Web access at ScienceDirect

I. Picek, R. Vianello, P. Sket, J. Plavec and B. Foretic
Tandem β-Elimination/Hetero-Michael Addition Rearrangement of an N-Alkylated Pyridinium Oxime to an O-Alkylated Pyridine Oxime Ether: An Experimental and Computational Study
J. Org. Chem. 2015, 80 (4), 2165–2173.
Web access at ACS publications

P. Galer, B. Wang, P. Sket and J. Plavec
Reversible pH Switch of Two-Quartet G-quadruplexes Formed by Human Telomere
Angew. Chem. Int. Ed. 2016, 55(6), 1993-1997.
Web access at Wiley Online Library.
Angew. Chem. 2016, 128 (6), 2033–2037.
Web access at Wiley Online Library.

LIST OF PUBLICATIONS

2022

O. Dlouhý, V. Karlický, U. Javornik, I. Kurasová, O. Zsiros, P. Šket, S. D. Kanna, K. Böde, K, Večeřová, O. Urban, E. S. Gasanoff, J. Plavec, V. Špunda, B. Ughy and G. Garab
Structural Entities Associated with Different Lipid Phases of Plant Thylakoid Membranes—Selective Susceptibilities to Different Lipases and Proteases
Cells 2022, 11 (17), 2681.
Web access at MDPI

HODŽIĆ, Aden, BIRARDA, Giovanni, JURAIĆ, Krunoslav, ŠKET, Primož, EDER, S., KRIECHBAUM, Manfred, DʹAMICO, F., DEGIACOMO, O., ROBLEGG, Eva
Revealing hidden molecular nanostructure details in the pellet formulation of ibuprofen by combining Synchrotron and laboratory sources. Journal of drug delivery science and technology
2022, vol. 68, str. 1-8, ilustr. ISSN 1773-2247
Web access at ScienceDirect

D. Pavc, N. Sebastian, L. Spindler, I. Drevenšek-Olenik, G. Koderman Podboršek, J. Plavec and P. Šket
Understanding self-assembly at molecular level enables controlled design of DNA G-wires of different properties
Nat. Commun. 2022, 13, article number: 1062.
Web access at Nature Communications

2021

O. Dlouhý, U. Javornik, O. Zsiros, P. Šket, V. Karlický, V. Špunda, J. Plavec and G. Garab
Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane—Particles. I. 31P-NMR Spectroscopy
Cells 2021, 10 (9), 2354.
Web access at MDPI

S. Guzelj, S. Nabergoj, M. Gobec, S. Pajk, V. Klančič, B. Slütter, R. Frkanec, A. Štimac, P. Šket, J. Plavec, I. Mlinarič-Raščan and Ž. Jakopin
Structural fine-tuning of desmuramylpeptide NOD2 agonists defines their in vivo adjuvant activity
J. Med. Chem. 2021, 64 (11), 7809-7838.
Web access at ACS Publications

2020

O. Dlouhý, I. Kurasová, V. Karlický, U. Javornik, P. Sket, N. Z. Petrova, S. B. Krumova, J. Plavec, B. Ughy, V. Spunda and G. Garab
Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluable enzyme violaxanthin de epoxidase
Sci. Rep. 2020, 10, article number: 11959.
Web access at nature.com

I. C. Ciobotaru , D. N. Crisan, P. Sket, C. C. Ciobotaru and S. Polosan
Detailed Molecular and Structural Analysis of Dual Emitter IrQ(ppy)₂ Complex
Materials 2020, 13, 1617
Web access at MDPI

D. Pavc, B. Wang, L. Spindler, I. Drevensek-Olenik, J. Plavec and P. Sket
GC ends control topology of DNA G-quadruplexes and their cation-dependent assembly
Nucleic Acids Res. 2020, 48 (5), 2749-2761.
Web access at Oxford Journals

T. Frelih, B. Wang, J. Plavec, and P. Sket
Pre-folded structures govern folding pathways of human telomeric G-quadruplexes
Nucleic Acids Res. 2020, 48 (4), 2189-2197.
Web access at Oxford Journals

T. Božič, M. Zalar, B. Rogelj, J. Plavec and P. Sket
Structural diversity of sense and antisense RNA hexanucleotide repeats associated with ALS and FTLD
Molecules 2020, 25(3), 525.
Web access at MDPI

2019

E. Ruggiero, S. Lago, P. Sket, M. Nadai, I. Frasson, J. Plavec and S. Richter
A dynamic i-motif with a duplex stem-loop in the long terminal repeat promoter of the HIV-1 proviral genome modulates viral transcription
Nucleic Acids Res. 2019, 47 (21), 11057-11068.
Web access at Oxford Journals

B. Ughy, V. Karlicky, O. Dlouhy, U. Javornik, Z. Materova, O. Zsiros, P. Sket, J. Plavec, V. Spunda, G. Garab
Lipid-polymorphism of plant thylakoid membranes. Enhanced non-bilayer lipid phases associated with increased membrane permeability
Physiol. Plantarum 2019, 166 (1), 278-287.
Web access at Wiley Online Library

M. Preskar, T. Vrbanec, F. Vrecer, P. Sket, J. Plavec and M. Gasperlin
Solubilization of ibuprofen for freeze dried parenteral dosage forms
Acta Pharm. 2019, 69, 17-32.
Web access at Acta Pharmaceutica

K. Klun, I. Falnoga, D. Mazej, P. Sket, J. Faganeli
Colloidal Organic Matter and Metal (loid) s in Coastal Waters (Gulf of Trieste, Northern Adriatic Sea)
Aquat. Geochem. 2019, 25(5-6), 179-194.
Web access at Springer

2018

N. D. Pajic, P. Djinovic, G. Dražić, J. Grdadolnik, P. Sket, J. Cerkovnik, A. Pintar
Structural stabilization and characterization of active peroxo species on TiO2-nanotube based materials in mild catalytic wet peroxide oxidation process
Appl. Catal. A. 2018, 562, 276-283
Web access at ScienceDirect

A. Romano, I. Roppolo, M. Giebler, K. Dietliker, S. Možina, P. Sket, I. Mühlbacher, S. Schlögl, M. Sangermano.
Stimuli-responsive thiol-epoxy networks with photo-switchable bulk and surface properties
RSC Adv. 2018, 8(73), 41904-41914
Web access at RCS Publishing

S. Lukin, M. Tireli, I. Loncaric, D. Barisic, P. Sket, D. Vrsaljko, M. di Michiel, J. Plavec, K. Uzarevic and I. Halasz
Mechanochemical carbon–carbon bond formation that proceeds via a cocrystal intermediate
Chem. Comm. 2018, 54(94), 13216-13219.
Web access at Natural Sciences Publishing

2017

T. Vrbanec, P. Sket, F. Merzel, M. Smrkolj, J. Grdadolnik.
Spectroscopic Characterization of Omeprazole and Its Salts
J. Spectrosc. 2017.
Web access at Hindawi

G. Garab, B. Ughy, P. de Waard, P. Akhtar, U. Javornik, C. Kotakis, P. Sket, V. Karlicky, Z. Materova, V. Spunda, J. Plavec, H. van Amerongen, L. Vigh, H. van As and P. H. Lambrev
Lipid polymorphism in chloroplast thylakoid membranes – as revealed by 31P-NMR and time-resolved merocyanine fluorescence spectroscopy
Sci. Rep. 2017, 7, article number: 13343.
Web access at nature.com

2016

S. Sersen, P. Sket, J. Plavec and I. Turel
Interactions of Two Cytotoxic Organoruthenium(II) Complexes with G-Quadruplex
J. Inorg. Biochem. 2016, 160, 70-77.
Web access at ScienceDirect

S. Roca, D. Vikic-Topic, J. Plavec, P. Sket, Z. Mihalic, D. Matkovic-Calogovic and Z. Popovic
Structural diversity of the Ag coordination sphere in complexes of silver(I) nitrate with 3-halopyridine. Characterization of the complexes in solution and in the solid state
Polyhedron 2016, 109, 166-175.
Web access at ScienceDirect.

2015

A. Kovanda, M. Zalar, P. Sket, J. Plavec and B. Rogelj
Anti-sense dDNA (GGCCCC)n expansions in C9ORF72 form i-motifs and protonated hairpins
Sci. Rep. 2015, 5, article number: 17944.
Web access at nature.com

K. Klun, P. Sket, I. Falnoga, J. Faganeli
Variation in colloidal organic matter composition and aggregation in coastal waters (Gulf of Trieste, Northern Adriatic Sea)
Geomicrobiol. J. 2015, 32(7), 609-615
Web access at taylor francis online

K. Burja, U. Segedin, S. Skale, P. Berce, P. Sket , P. Prosen ,D. Kukanja
Improved anticorrosion properties of polyurethane coatings based on high-solids acrylics synthesized in a high pressure reactor
Prog. Org. Coat. 2015, 78, 275-286
Web access at Bentham Science

A. Kotar, M. Kotar, P. Sket and J. Plavec
Potential of NMR and SEM in Characterization of Tablets of Ibuprofen
Curr. Pharm. Anal. 2015, 11(2), 124-130.
Web access at ScienceDirect

2014

M. Juribasic, N. Bregovic, V. Stilinovic, V. Tomisic, M. Cindric, P. Sket, J. Plavec, M. Rubcic and K. Uzarevic
Supramolecular stabilization of metastable tautomers in solution and solid state
Chem Eur J. 2014, 20 (52), 17333-17345.
Web access at Wiley Online Library

P. Sket, T. Korbar and J. Plavec
Influence of 3’-3’ Inversion of Polarity Site within d(TGGGGT) on Inter Quartet Cation Binding
J. Mol. Struc. 2014, 1075, 49-52.
Web access at Elsevier

M. Rubcic, N. Galic, I. Halasz, T. Jednacak, N. Judas, J. Plavec, P. Sket and P. Novak
Multiple solid forms of 1,5-bis(salicylidene)carbohydrazide: polymorph-modulated thermal reactivity
Cryst. Growth Des. 2014, 14 (6), 2900–2912.
Web access at ACS publications

S. Ceru, P. Sket, I. Prislan, J. Lah and J. Plavec
A new pathway of DNA G-quadruplex formation
Angew. Chem. Int. Ed. 2014, 53 (19), 4881-4884.
Web access at Wiley Online Library

T. Jednacak, P. Novak, A. Hodzic, O. Scheibelhofer, J. G. Khinast, J. Plavec, P. Sket and J. Parlov Vukovic
Condensation Reaction between Carbohydrazide and Salicylaldehyde: in-line Vibrational Spectroscopy Monitoring and Characterization of the Reaction Products in Solution and Solid state
Acta Chim. Slov. 2014, 61 (1), 161-169.
Web access at Acta Chimica Slovenica

M. Zegarac, E. Leksic, P. Sket, J. Plavec, M. Devcic Bogdanovic, D.-M. Bucar, M. Dumic, E. Mestrovic
A sildenafil cocrystal based on acetylsalicylic acid exhibits an enhanced intrinsic dissolution rate
CrystEngComm 2014, 16(1), 32-35.
Web access at RSC Publishing

2013

T. Ilc, P. Sket, J. Plavec, M. Webba da Silva, I. Drevensek Olenik, L. Spindler
Formation of G-wires : the role of G:C-base pairing and G-quartet stacking
J. Phys. Chem. C, Nanomaterials and interfaces, 2013, 117 (44), 23208-23215.
Web access at ACS publications

K. Tluckova, M. Marusic, P. Tothova, L. Bauer, P. Sket, J. Plavec and V. Viglasky
Human Papillomavirus G-Quadruplexes
Biochemistry 2013, 52 (41), 7207-7216.
Web access at ACS publications

M. Necemer, P. Kump, P. Sket, J. Plavec, J. Grdadolnik and M. Zvanut
A Novel Analytical Technique Suitable for the Identification of Plastics
Acta Chim. Slov. 2013, 60 (3), 701-705.
Web access at Acta Chimica Slovenica

2012

P. Sket, W. Kozmiński and J. Plavec
Is there any Proton Exchange between Ammonium Ions localized within the d(G3T4G4)2 Quadruplex?
Acta Chim. Slov. 2012, 59 (3), 473–477.
Web access at Acta Chimica Slovenica

P. Sket, A. Virgilio, V. Esposito, A. Galeone and J. Plavec
Strand directionality affects cation binding and movement within tetramolecular G-quadruplexes
Nucl. Acids Res. 2012, 40 (21): 11047-11057.
Web access at Oxford Journals

Y. Hua, P. Changenet-Barret, R. Improta, I. Vayá, T. Gustavsson, A.B. Kotlyar, D. Zikich, P. Sket, J. Plavec and D. Markovitsi
Cation Effect on the Electronic Excited States of Guanine Nanostructures Studied by Time-Resolved Fluorescence Spectroscopy
J. Phys. Chem. C 2012, 116 (27), 14682-14689.
Web access at ACS publications

M. Marusic, P. Sket, L. Bauer, V. Viglasky, and J. Plavec
Solution-state structure of an intramolecular G-quadruplex with propeller, diagonal and edgewise loops
Nucleic Acids Res. 2012, 40 (14), 6946-6956.
Web access at Oxford Journals

V. Strukil, I. Dzilovic, D. Matkovic-Calogovic, J. Saame, I. Leito, P. Sket, J. Plavec and M. Eckert-Maksic
Molecular structure and acid/base properties of 1,2-dihydro-1,3,5-triazine derivatives
New J. Chem. 2012, 36 (1), 86-96.
Web access at RCS Publishing

2011

P. Sket, R. Pirh and J. Plavec
Do anions exert any structural changes on dimeric d(G4T4G4)2 quadruplex in aqueous solution?
IReBiC 2011, 2 (3), 68-73.
praise worthy prize

D. Cincic, M. Juribasic, D. Babic, K. Molcanov, P. Sket, J. Plavec and M. Curic
New Insight into Solid-State Molecular Dynamics: Mechanochemical Synthesis of Azobenzene/Triphenylphosphine Palladacycles
Chem. Commun. 2011, 47 (41), 11543-11545.
RSC Publishing

J. Seliger, J. Plavec, P. Sket, V. Zagar and R. Blinc
¹⁷O NQR and ¹³C NMR study of hydrogen-bonded organic ferroelectric croconic acid
Phys. Status Solidi B 2011, 248 (9), 2091–2096.
Web access at Wiley Online Library

2010

J. Kastelic, Z. Hodnik, P. Sket, J. Plavec, N. Lah, I. Leban, M. Pajk, O. Planinsek and D. Kikelj
Fluconazole Cocrystals with Dicarboxylic Acids
Cryst. Growth Des., 2010, 10 (11), 4943-4953.
Web access at ACS Publications

P. Sket and J. Plavec
Tetramolecular DNA Quadruplexes in Solution: Insights into Structural Diversity and Cation Movement
J. Am. Chem. Soc., 2010, 132 (36), 12724-12732.
Web access at ACS Publications

2009

M. Trajkovski, P. Sket and J. Plavec
Cation Localization and Movement within DNA Thrombin Binding Aptamer in Solution
Org. Biomol. Chem. 2009, 7 (22), 4677-4684.

2008

P. Podbevsek, P. Sket and J. Plavec
Stacking and Not Solely Topology of T3 Loops Controls Rigidity and Ammonium Ion Movement within d(G₄T₃G₄)₂ G-Quadruplex
J. Am. Chem. Soc. 2008, 130 (43), 14287-14293.

2007

P. Podbevsek, P. Sket and J. Plavec
NMR study of ammonium ion binding to d[G₃T₄G₄]₂ and d[G₄(T₄G₄)₃] G-quadruplexes
Nucl. Nucl. and Nucl. Acids 2007, 26 (10), 1547-1551.

2005

P. Sket, M. Crnugelj and J. Plavec
Identification of mixed di-cation forms of G-quadrupex in solution
Nucleic Acids Res. 2005, 33 (11), 3691-3697.

2004

P. Sket , M. Crnugelj, W. Kozminski and J. Plavec
¹⁵NH₄⁺ ion motion inside d(G₄T₄G₄)₂ G-quadruplex is accelerated in the presence of smaller Na⁺ ions
JOrg. Biomol. Chem. 2004, 2 (14), 1970-1973.

P. Sket, M. Crnugelj and J. Plavec
d(G₃T₄G₄) forms unusual dimeric G-quadruplex structure with the same general fold in the presence of K⁺, Na⁺ or NH₄⁺ ions
JOrg. Biomol. Chem. 2004, 2 (14), 1970-1973.

2003

M. Crnugelj, P. Sket and J. Plavec
Small change in a G-rich sequence, a Dramatic Change in Topology: New Dimeric G-quadruplex Folding Motif with Unique Loop Orientations
J. Am. Chem. Soc. 2003, 125 (26), 7866-7871.