Oro, a new 600 MHz NMR spectrometer

We are proud to announce that Oro, a new Bruker 600 MHz NMR spectrometer has arrived!
Take a quick look at just a few pictures of Oro's arrival below or view the full gallery of Oro's installation process HERE.

More pictures of the ongoing Oro's installation will be added, so stay tuned.

20. 5. 2020

Nucleic acid structure webinar

Enlarge the picture for more details about the webinar.

For registration, please send an email to G4webinarseries@gmail.com

12. 5. 2020

Fascinating new publication in Nucleic Acids Research journal

In a search for a predictable way of (de)stabilizing G-quadruplex structures we have investigated the effect of pyrene-modified uridine nucleotides incorporated into the thrombin binding aptamer (TBA) model system. Characterization using spectroscopic and biophysical methods provided important insights into modes of interaction between pyrene groups and the G-quadruplex core as well as (de)stabilization by enthalpic and entropic contributions. NMR data demonstrated that incorporation of pyrene group into G-rich oligonucleotide such as TBA may result in significant changes in 3D structure such as formation of a novel dimeric topology. Site-specific structural changes induced by stacking of the pyrene moiety on nearby nucleobases correlate with distinct thrombin binding affinities and increased resistance against nuclease degradation.

We also congratulate Matic Kovačič on his first paper, published in the prestigious Nucleic Acids Research journal.

For more information read the original paper: here

14. 4. 2020

Another great publication in Nucleic Acids Research journal

Congratulations to Daša Pavc on her first paper, published in the prestigious Nucleic Acids Research journal.
The paper describes high-resolution G-quadruplex structures formed by DNA oligonucleotides GCn and GCnCG in the presence of Na⁺ ions. As revealed by NMR spectroscopy, 5’-GC ends have crucial role in formation of these G-quadruplexes with unprecedented folding topologies. Change of Na⁺ ions for ¹⁵NH₄⁺ and K⁺ ions induces 3’-3’ stacking via terminal G-quartets of GCn G-quadruplexes, which is precluded by 3’-GC overhangs in the case of GCnCG. Results of the present study expand repertoire of possible G-quadruplex structures.

For more information read the original paper: here

9. 3. 2020